Table 1.
Experimental and control groups used in the current study with indication of age of animal, period of visual deprivation and treatment of tissue for immunofluorescence.
| Control | Visual deprivation | Fixation (1% PFA) |
|---|---|---|
| E12.5, 14.5, 20.5 | Immersion | |
| P1, P3, P7 | 1 week (P0–7) | Immersion |
| P10 | Perfusion | |
| P15 | From birth (P0–15) | Perfusion |
| P21 | Perfusion | |
| P28 | From birth (P0–28) | Perfusion |
| 1 week (P21–28) | ||
| 2 weeks (P14–28) | ||
| P35 | From birth (P0–35) | Perfusion |
| 2 weeks (P21–35) | ||
| >P55, >P180 | Perfusion |
E, embryonic; P, postnatal; PFA, paraformaldehyde.