Table 2.
Specification of antibodies with indication of catalog number, clone, working dilution, previously conducted controls, sources and references where available, and the corresponding figure in the manuscript.
| Antibody catalog No. Clone/type | Dilution in IF and WB | Reported specificity | Source references | Figures | ||
|---|---|---|---|---|---|---|
| KO | IHC | WB | ||||
| α/β tubulin (rb) #2148 | WB: 1:1000 | X | X | Cell signaling, Frankfurt, Germany Marini et al., 2006 | Figures 5C,E,F | |
| Ankyrin-G (rb) sc-28561 H-215 | IF: 1:500 WB: 1:300 | X | X | X | Santa Cruz, Heidelberg, Germany Engelhardt et al., 2013 | Figures 1A,D, 2B,D–G, 3B–C1, 5A–C,E, S1A,C,E |
| Ankyrin-G (ms) 73-146 N106/36 | IF: 1:500 | X | X | X | UC Davis/NIH NeuroMab Facility, CA, USA Engelhardt et al., 2013 | Figures 2C, S1A,C,E, S2A,C,E |
| βIV spectrin (rb) amino acids 2237–2256 of human βIV spectrin | IF: 1:500 | X | X | X | Selfmade Berghs et al., 2000 | Figures 1B, S2A,C,E |
| Myelin basic protein (rt), ab7349, clone 12 | IF: 1:1000 WB: 1:15000 | X | AbCam, Cambridge, UK Engelhardt et al., 2013 | Figures 5A,B,E | ||
| NeuN (ms) MAB377, clone A60 | IF: 1:500 | X | X | Millipore, Temecula, USA Engelhardt et al., 2007 | Figures 1A,B, 3B,C1 | |
| panNaV (ms) S8809 clone K58/35 | IF: 1:500 | X | X | Sigma, Saint Louis, MO, USA Rasband et al., 1999 | Figures 1C,D, 5F | |
| reelin (ms) MAB5364, clone G10 | IF: 1:500 | X | X | Millipore, Billerica, USA Doehner et al., 2012 | Figure 2D | |
IF, immunofluorescence; IHC, immunohistochemistry; IP, immunoprecipitation; KO, lack of signal in knock out animals; WB, western blot; rb, rabbit; ms mouse; rt, rat.