Table 2. Intra-individual stability of IgG glycans, given as coefficients of variation of all HILIC-UPLC separated glycan groups measured through four days in nine individuals.
Glycan group | Coefficient of variation (%) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Person 1 | Person 2 | Person 3 | Person 4 | Person 5 | Person 6 | Person 7 | Person 8 | Person 9 | average | reproducibility of the analytical method* | |
GP1 | 12.6 | 9.1 | 12 | 16.7 | 20 | 0.09 | 9.1 | 8.4 | 14.9 | 11.5 | 26.7 |
GP2 | 5.06 | 8.6 | 8.9 | 4.56 | 3.6 | 2.77 | 3.8 | 8.1 | 5.22 | 5.62 | 9.97 |
GP4 | 1.16 | 0.9 | 3.2 | 2.06 | 2.6 | 3.39 | 3.7 | 1.1 | 3.07 | 2.34 | 7.92 |
GP5 | 7.01 | 2.9 | 21 | 5.52 | 8 | 6.34 | 6.3 | 7.8 | 8.3 | 8.18 | 6.37 |
GP6 | 0.94 | 1.1 | 4.2 | 0.49 | 3.4 | 1.6 | 3.9 | 1.5 | 2.91 | 2.23 | 11.6 |
GP7 | 10.6 | 2.6 | 7.5 | 4.28 | 4 | 4.38 | 5.1 | 1.2 | 10.6 | 5.59 | 4.44 |
GP8 | 0.94 | 0.8 | 2 | 0.66 | 0.7 | 0.74 | 1.9 | 1.1 | 1.09 | 1.09 | 9.54 |
GP9 | 0.45 | 0.4 | 4.7 | 1.78 | 2.1 | 2.01 | 2.6 | 2.6 | 3.48 | 2.23 | 3.06 |
GP10 | 2.35 | 1.3 | 1.6 | 0.18 | 1.2 | 1.75 | 2.9 | 2.2 | 0.39 | 1.54 | 2.63 |
GP11 | 6.14 | 1.6 | 4.4 | 4.59 | 2.1 | 0.98 | 3.4 | 3.2 | 6.21 | 3.63 | 2.76 |
GP12 | 1.21 | 2.9 | 13 | 5.06 | 5 | 4.16 | 0.9 | 8.1 | 2.17 | 4.73 | 4.95 |
GP13 | 6.51 | 5.8 | 15 | 0.97 | 10 | 18.3 | 3.3 | 9.6 | 18.8 | 9.84 | 6.55 |
GP14 | 1.14 | 0.5 | 1.3 | 1.33 | 1 | 0.81 | 0.7 | 1.3 | 1.67 | 1.08 | 7.58 |
GP15 | 0.85 | 0.5 | 1.5 | 1.56 | 1.9 | 2.15 | 0.8 | 1.1 | 3.78 | 1.59 | 3.26 |
GP16 | 1.24 | 3.6 | 1.1 | 1.93 | 3.5 | 3.63 | 5.3 | 5.9 | 1.83 | 3.11 | 4.22 |
GP17 | 4.05 | 1.8 | 4.7 | 6.19 | 4 | 3.16 | 6.7 | 3.7 | 8.15 | 4.72 | 3.33 |
GP18 | 1.05 | 0.7 | 3.5 | 0.68 | 1.9 | 1.4 | 3.3 | 2.9 | 3.66 | 2.11 | 6.02 |
GP19 | 2.45 | 2.4 | 5.1 | 3.86 | 3.1 | 3.07 | 7.5 | 3.3 | 7.95 | 4.31 | 4.43 |
GP20 | 7.73 | 5 | 4.9 | 15.6 | 9.7 | 10.1 | 30 | 7.3 | 6.5 | 10.7 | 5.08 |
GP21 | 11.1 | 8.9 | 29 | 13 | 14 | 8.25 | 24 | 13 | 12.7 | 14.8 | 8.54 |
GP22 | 15.7 | 10 | 21 | 4.56 | 40 | 12.1 | 37 | 21 | 13.4 | 19.4 | 15.9 |
GP23 | 3.72 | 7.4 | 10 | 0.74 | 5.5 | 3.6 | 26 | 8 | 8.2 | 8.18 | 14.2 |
GP24 | 5.97 | 8.1 | 8.7 | 0 | 3.6 | 5.58 | 22 | 12 | 5.31 | 7.9 | 9.08 |
average | 4.78 | 3.76 | 8.25 | 4.18 | 6.56 | 4.37 | 9.12 | 5.81 | 6.53 | 5.93 | 8.65 |
*determined by ten repeated analysis of the same sample (IgG isolation, glycan release and labelling, HPLC analysis) on different occasions.