FIGURE 1.

Calorimetric titrations of VLRB.aGPA.23 with glycan ligands at 10 °C. A, raw data were obtained from 48 automatic injections of 0.5-μl aliquots of 5.02 mm TFα solution into 0.370 mm VLRB.aGPA.23 solution. B, shown is the incremental heat per mole of added ligand (open squares) for the titration in A. The K_b obtained from this titration is 2.21 ± 0.06 × 10⁴ m⁻¹ with n = 1.09 ± 0.01. C, raw data were obtained from 48 automatic injections of 0.5-μl aliquots of 4.80 mm TFα-Ser solution into 0.370 mm VLRB.aGPA.23 solution. D, shown is the incremental heat per mole of added ligand (open squares) for the titration in C; K_b = 2.04 ± 0.06 × 10⁴ m⁻¹ with n = 0.97 ± 0.10. E, raw data were obtained from 19 automatic injections of 2-μl aliquots of 7.4 mm BG-H solution into 0.310 mm VLRB.aGPA.23 solution. F, shown is a non-linear least-squares fit (solid line) of the incremental heat per mole of added ligand (open squares) for the titration in E; K_b = 2.04 ± 0.06 × 10⁴ m⁻¹ with n = 1.06 ± 0.07.