Figure 10. Transgenic over-expression of the short transcript from the cDNA library has no effect on expression of BCL2L11.

The vector from the cDNA library with the insert from Figure 1 was used for tranfection of L-428 cells. RNA was isolated 48 h after transfection. Quantitative RT-PCR was used for determination of expression of the transgene (ERVK_1q42.13), BCL2L11, DUSP5P1, and DUSP5, respectively. For calculation of relative expression values, GAPDH was used as housekeeping control and the mean Δct value from control cells was set as 1. Presented are means and standard deviations from 3 independent experiments. With exception of ERVK_1q42.13 (p<10⁻⁶), all other differences are statistically not significant.