FIG. 2.
Iodine influence on Notch signaling. (A) High iodine treatment decreased Notch1 protein in BRAF-activated thyroid cells analyzed by Western blot. (B) BRAF oncogene activation increased Notch signaling through Notch-CBF1 binding to CBF plasmid and enhanced luciferase activity. Iodine treatment blocked BRAF-induced Notch signaling activation in PC-BRAFV600E-6 cells. Mutated CBF binding site plasmid (mCBF) was used as binding specificity control. Luminescence (arbitrary units, a.u.) was normalized by cotransfection of pRL-CMV plasmid that encodes Renilla luciferase. *p<0.05 vs. Control. Data are expressed as mean±SE (n=3). Representative results of two independent experiments.