FIG. 3.
Effect of Notch1 inhibition on miR-19 expression. (A, B) Notch1 siRNA treatment during 48-hour BRAF activation in PC-BRAFV600E-6 cells reduced Notch1 mRNA and inhibited miR-19a and miR-19b. (C, D) Knockdown of NOTCH1 in KTC2 cells resulted in decreased levels of miR-19a and miR-19b after 48 hours transfection. *p<0.05, **p<0.01 vs. siRNA EGFP. (E) BRAFV600E inhibition with specific inhibitor PLX4032 (1 μM for 24 hours) resulted in reduction of miR-19 levels in KTC2 cells. Data are expressed as mean±SE (n=3). *p<0.05 vs. DMSO.