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. Author manuscript; available in PMC: 2014 Mar 12.
Published in final edited form as: J Am Chem Soc. 2013 Dec 13;135(51):19260–19267. doi: 10.1021/ja409608r

Figure 4.

Figure 4

FTIR melt curves for the FBP28 1L (red) and FBP28 2L (blue) WW domains obtained by plotting the change in IR difference spectra at 1634 cm−1 versus temperature. The data are fit to an apparent 2-state model (eq 2) and then normalized. Inset: van’t Hoff analyses using equilibrium constants generated from the melt curves.