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. 2014 Mar 1;24(3):502–510. doi: 10.1089/thy.2013.0038

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 1. — Effect of TshR knockout on mRNA levels of transgene and thyroid-specific genes. Total RNA was extracted from the thyroids at 12 weeks and subjected to quantitative reverse-transcription polymerase chain reastion (qRT-PCR) for the expression of the indicated genes after normalization to β-actin. Columns represent means and standard error of the mean (SEM) of values obtained from three independent biological replicates. 1, group 1 (Braf^wt/TshR^+/−); 2, group 2 (Braf^wt/TshR^−/−); 3, group 3 (Tg-BRAF2/TshR^+/−); 4, group 4 (Tg-BRAF2/TshR^−/−).