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. 2014 Mar 15;31(6):582–594. doi: 10.1089/neu.2013.3146

FIG. 1.

FIG. 1.

Valproic acid (VPA) inhibits cell death of ventral motor neurons (VMN) after spinal cord injury (SCI). VPA (300 mg/kg) was injected subcutaneously into the rats after SCI and spinal tissues were isolated at 1 day for Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) and 3 days for Nissl staining and counting (n=5/group). (A) Representative Nissl staining showing ventral horn of spinal cord 2 mm rostral from lesion site. Scale bar, 50 μm. (B) Counting analysis of VMN from 4 mm to 4 mm rostral and caudal from lesion site. (C) Representative TUNEL staining with injured spinal tissue 1 mm rostral from lesion site. Scale bar, 30 μm. (D) Quantification of TUNEL-positive cells in the ventral horn from 2 mm to 2 mm rostral and caudal from lesion site. Note that VPA significantly inhibited VMN cell loss and cell death after SCI. Data represent the mean±SD. *p<0.05.