Table 3. Molecular size and putative oligomeric state of wild-type and mutant BGLPf.

The theoretical molecular mass of monomeric BGLPf estimated from the amino-acid sequence is 55 kDa. Therefore, the gel filtration suggests that BGLPf-WT is tetrameric, BGLPf-M3 is dimeric and BGLPf-M4a and BGLPf-M4c are monomeric. These data are also supported by the DLS measurements except for BGLPf-M3, which does not show a dimeric size.

	BGLPf-WT	BGLPf-M3	BGLPf-M4a	BGLPf-M4b	BGLPf-M4c
Molecular mass (gel filtration) (kDa)	236	136	71	96	50
Radius (DLS) (nm)	5.7 ± 1.1	16.8 ± 19.2	4.0 ± 1.7	4.1 ± 1.6	3.2 ± 1.0
Putative oligomeric state	Tetramer	Dimer	Monomer	Dimer or monomer	Monomer