Figure 3.

Effect of prime-boost bacille Calmette–Guérin (BCG)/DNAhsp65 in the insulitis score in non-obese diabetic (NOD) mice. The degrees of insulitis (a) were evaluated using a semi-quantitative scale: (i) 0 = intact islet; (ii) 1 = peri-insulitis; (iii) 2 = moderate insulitis (< 50% mononuclear infiltration); and (iv) 3 = severe insulitis (more than 50% mononuclear infiltration). The analysis of the severity of mononuclear infiltration in NOD immunized with the prime boost BCG/DNAhsp65 is shown in (c) based on the score described above. All islets of all pancreas sections were analysed.