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. 2013 Aug 6;173(3):454–462. doi: 10.1111/cei.12122

Figure 4.

Figure 4

(a) Effects of IL-6 and B-MSC supernatants on CD4+ lymphocytes enriched in Tregs (CD4+CD25+CD127−). d0: baseline Treg percentage in CD4+ cultures before experiments. d5: Treg percentage in lymphocyte cultures without media supplementation. d5 + 5 ng/ml/d5 + 10 ng/ml: Treg percentage in lymphocyte cultures with 5 or 10 ng/ml IL-6 supplementation. d5 + MSC supernatant: Treg percentage in lymphocyte cultures with supplementation of B-MSC cultures derived supernatants. **P < 0.01, ***P < 0.001. (b) Effects of IL-6 and B-MSC supernatants on CD4+ lymphocytes enriched in Tregs. No significant differences in the percentage of CD4+ were observed between the groups.