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The poly(I:C) effect on lipocalin 2 (LCN2) release was silenced in HT-29 cells by transfection with Toll-like receptor (TLR)-3 siRNA. The cells were transfected for 24 h using TLR-3 siRNA or control/non-signalling siRNA and then stimulated with poly(I:C) or left unstimulated (medium) for 20 h. * and #P < 0·05. Mean ± s.d. of triplicates.
