Reactivity of W6/32 and HC10 on different bead preparations. Regular single antigen bead (SAB) (a), denatured SAB coated with denatured human leucocyte antigen (HLA) (b) and iBeads (c) were incubated with W6/32 or HC10. The beads used to assess reactivity against denatured HLA (b) were treated with acid, as described in the Patients and methods section. Shown are the raw mean fluorescence intensity (MFI) values with those of W6/32 on the x-axis and HC10 on the y-axis. Specificities found by HC10 > 1000 MFI on iBeads were B*44:03, B*47:01, B*15:11, B*39:01, A*66:01, B*18:01, B*51:02, A*69:01, A*33:03, B*15:10, A*33:01, B*15:16, A*34:02, B*15:02, A*66:02, A*25:01, B*13:01, B*15:13, B*37:01, A*34:01, A*68:02, A*26:01, B*35:01, B*53:01 and B*38:01.