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. 2013 Aug 6;173(3):536–543. doi: 10.1111/cei.12127

Figure 2.

Figure 2

Comparison of mean fluorescence intensity (MFI)-values and donor-specific human leucocyte antigen (HLA) antibodies (DSA) between different bead preparations. Raw MFI-values of single antigen bead (SAB) analysis, iBeads and beads coated with denatured HLA from 156 sera were obtained by testing on the same lot number of single antigen beads. (a) Shows > 15·000 luminex data points comparing SAB with iBeads and (b) contains the same number of comparisons between SAB and beads with denatured HLA. Cut-off reference lines discriminating positive from negative reactions (as indicated in the Patients and methods section) are indicated in (a) and (b) by striped lines. The solid lines in (a) and (b) show linear regressions including the R2 and P-values. MFI values of DSA assigned by the different bead preparations are depicted in (c), whereas the cumulative MFI values of all DSA found in individual sera are shown in (d). The lines in panels (c) and (d) represent average values found.