Figure 9.

Confocal images showing the localization of nNOS-IR and MrgC receptor IR in DRG neurons. nNOS-IR neurons are identified by FITC fluorescence (A, arrows, green) whereas MrgC receptor-IR-positive neurons by rhodamine (B, arrows, red). nNOS-IR is extensively co-localized with MrgC receptor-IR in DRG neurons (C, arrows, yellow). Incubation of DRG section with anti-MrgC receptor antiserum preabsorbed with 1 μM MrgC receptor protein resulted in the complete absence of staining (D). Scale bar = 50 μm.