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Effect of PGG on the binding of LPS to TLR-4 on peritoneal macrophages. Peritoneal macrophages (5 × 105 cells) isolated from mice were incubated with Alexa Fluor 488-conjugated LPS for 20 min in the absence or presence of PGG (2 or 5 μM), and then analysed by flow cytometry (A), confocal microscopy (B), and immunoblotting (C). TLR4 and β-actin were analysed by immunoblotting.
