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Recombinant human FSH treatment resulted in elevated Gdnf mRNA levels in 1-week co-cultures started from A) adult and B) juvenile seminiferous tubule fragments. In juvenile mouse-derived co-cultures FSH treatment was followed by an acute increase in Gdnf mRNA levels at 6 hours. Retreatment 72 hours after initial dose lead to increased levels 24 hours later. White bars, control; black bars, 10 ng/ml rhFSH; n = 3, SEM; *, p<0.05; **, p<0.01.
