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. 2014 Mar 11;9(3):e90088. doi: 10.1371/journal.pone.0090088

Figure 9. Quantitative RT-PCR analysis of somatic cell-specific marker expression in starting material and 1–5-week co-cultures.

Figure 9

A) Relative αSMA mRNA levels were increased in the beginning of the culture, whereas WT1 levels displayed the opposite. B) Fibroblast marker Fsp1 was found at a very high level from week 1 onwards. C) Endothelial cell marker mRNA levels were acutely increased (Cdh5 and CD141) or did not change (vWF) during the course of 5 weeks. D) Messenger-RNAs associated with juvenile, immature Sertoli cells (Krt18 and Pdpn) were found at their highest level in 1–2 week co-cultures. n = 3, SEM; *, p<0.05; **, p<0.01, ***, p<0.001.