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. 2014 Mar 11;9(3):e90088. doi: 10.1371/journal.pone.0090088

Table 2. Primer design, annealing temperatures and PCR product lengths of the studied mRNAs.

Gene Accession number Annealing temperature Primers Product length
αSMA NM_007392 62°C 5′-TGCTGTCCCTCTATGCCTCT-3′ 5′-GAAGGAATAGCCACGCTCAG-3′ 184 bp
CD141 NM_009378 55°C 5′- TCTGCGAGCATTTTTGTGTC-3′ 5′-GCACTCTCCATCCACCAACT-3′ 215 bp
CD9 NM_007657 56°C 5′-TGCAGTGCTTGCTATTGGAC-3′ 5′-GGCGAATATCACCAAGAGGA-3′ 219 bp
Cdh5 NM_009868 62°C 5′-ATTGAGACAGACCCCAAACG-3′ 5′-TTCTGGTTTTCTGGCAGCTT-3′ 238 bp
CIP2A NM_172616 57°C 5′-GCGCCATGTACTCAGTCAGA-3′ 5′-AGGAAGCAGAAGGGTCACAA-3′ 234 bp
c-Kit NM_021099 56°C 5′-ATCCCGACTTTGTCAGATGG-3′ 5′-AAGGCCAACCAGGAAAAGTT-3′ 192 bp
Erm NM_023794 57°C 5′-CAAGAGCCCCGAGATTACTG-3′ 5′-CTCGGGTACCACGCAAGTAT-3′ 146 bp
Fsp1 NM_011311 64°C 5′-ACTCAGGCAAAGAGGGTGAC-3′ 5′-TGCAGGACAGGAAGACACAG-3′ 188 bp
Gdnf NM_010275 61°C 5′-CGGACGGGACTCTAAGATGA-3′ 5′-CGTCATCAAACTGGTCAGGA-3′ 209 bp
Gfr1α NM_010279 63°C 5′-TGTACTTCGTGCTGCCACTC-3′ 5′-GCTGAAGTTGGTTTCCTTGC-3′ 166 bp
Gpr125 XM_991709 59°C 5′-GACCTGACGAACAACCGAAT-3′ 5′-CTGGTGTCTCGCACAGTGAT-3′ 235 bp
Krt18 NM_010664 55°C 5′-CGAGGCACTCAAGGAAGAAC-3′ 5′-CTTGGTGGTGACAACTGTGG-3′ 246 bp
L19 NM_009078 55°C 5′-GGACAGAGTCTTGATGATCTC-3′ 5′-CTGAAGGTCAAAGGGAATGTG-3′ 195 bp
Nanog NM_028016 64°C 5′-CCAGTGGAGTATCCCAGCAT-3′ 5′-GAAGTTATGGAGCGGAGCAG-3′ 236 bp
Pdpn NM_010329 58°C 5′-GCCAGTGTTGTTCTGGGTTT-3′ 5′-AGAGGTGCCTTGCCAGTAGA-3′ 191 bp
PLZF NM_001033324 59°C 5′-AACGGTTCCTGGACAGTTG-3′ 5′-CCCACACAGCAGACAGAAGA-3′ 172 bp
Ppia NM_008907 63°C 5′-CATCCTAAAGCATACAGGTCCTG-3′ 5′-TCCATGGCTTCCACAATGTT-3′ 164 bp
Stra8 NM_009292 57°C 5′-ATGCAATGTTGCTGAAGTGC-3′ 5′-GGAAGCAGCCTTTCTCAATG-3′ 161 bp
Sycp3 NM_011517 60°C 5′-AGCCAGTAACCAGAAAATTGAGC-3′ 5′-CCACTGCTGCAACACATTCATA-3′ 106 bp
vWF NM_011708 63°C 5′-GGCAAGAGAATGAGCCTGTC-3′ 5′-AAGCCAAAGGTCTCACTGGA-3′ 172 bp
WT1 NM_144783 64°C 5′-AGGTTTTCTCGCTCAGACCA-3′ 5′-GCTGAAGGGCTTTTCACTTG-3′ 160 bp