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. 2014 Mar 5;7:149–158. doi: 10.2147/IJGM.S45839

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© 2014 Tomblin et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Materials, preparation, and observation process flow chart.

Note: HEK-Bluet™-2 and QUANTI-Blue™ (InvivoGen, San Diego, CA, USA).

Abbreviations: LPS, lipopolysaccharide; MDP, muramyl dipeptide; NOD2-WT, nucleotide oligomerization domain 2-Wild Type; PMA, phorbol 12-myristate 13-acetate; SEAP, secreted alkaline phosphatase; Pam3CSK4, Pam3CysSerLys4; FSL-1, Pam2CGDPKHPKSF.