Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Time (min) | Flow (mL/min) | A (%) | B (%) |
| 0 | 0.25 | 100 | 0 |
| 0.5 | 0.25 | 100 | 0 |
| 20 | 0.25 | 5 | 95 |
| 21 | 0.25 | 5 | 95 |
| 22 | 0.25 | 100 | 0 |
| 25 | 0.25 | 100 | 0 |
A: water (0.1% formic acid), B: acetonitrile (0.1% formic acid).
