Table 1. Carbonate chemistry at the beginning, end and through (average) the experiments.
Culture | Treatment | pCO2 (µatm) | Avg pCO2 (µatm) | TA (µmol kg-1) | pHt | HCO3 − (µmol kg-1) | CO3 2− (µmol kg-1) | CO2 (µmol kg-1) | DIC (µmol kg-1) |
Initial | 1 | 426 | 2370 | 8.030 | 1899 | 188 | 13.7 | 2100.44 | |
2 | 786 | 2364 | 7.799 | 2062 | 120 | 25.3 | 2207.40 | ||
3 | 1709 | 2361 | 7.490 | 2201 | 63 | 54.9 | 2318.92 | ||
4 | 4637 | 2351 | 7.073 | 2284 | 25 | 149.0 | 2457.91 | ||
Final | 1 | 216 | 321 | 2397 | 8.270 | 1678 | 289 | 6.9 | 1973.72 |
1 | 320 | 373 | 2302 | 8.121 | 1761 | 215 | 10.3 | 1986.71 | |
1 | 329 | 377 | 2385 | 8.125 | 1825 | 225 | 10.6 | 2059.96 | |
1 | 331 | 378 | 2365 | 8.120 | 1814 | 221 | 10.6 | 2045.79 | |
2 | 329 | 558 | 2392 | 8.126 | 1831 | 226 | 10.6 | 2068.19 | |
2 | 329 | 558 | 2386 | 8.125 | 1827 | 225 | 10.6 | 2062.94 | |
2 | 550 | 668 | 2370 | 7.936 | 1977 | 158 | 17.7 | 2151.83 | |
2 | 561 | 674 | 2366 | 7.927 | 1978 | 155 | 18.0 | 2150.59 | |
2 | 587 | 687 | 2370 | 7.911 | 1994 | 150 | 18.9 | 2162.75 | |
3 | 815 | 1262 | 2369 | 7.787 | 2075 | 117 | 26.2 | 2218.75 | |
3 | 855 | 1282 | 2368 | 7.767 | 2085 | 113 | 27.5 | 2225.04 | |
3 | 1186 | 1447 | 2357 | 7.637 | 2141 | 86 | 38.1 | 2264.52 | |
3 | 1191 | 1450 | 2353 | 7.635 | 2138 | 85 | 38.3 | 2261.76 | |
3 | 1241 | 1475 | 2367 | 7.621 | 2157 | 83 | 39.9 | 2279.91 | |
4 | 2052 | 3345 | 2360 | 7.415 | 2224 | 53 | 65.9 | 2343.59 | |
4 | 2072 | 3354 | 2359 | 7.411 | 2224 | 53 | 66.6 | 2343.96 | |
4 | 2145 | 3391 | 2360 | 7.397 | 2229 | 51 | 68.9 | 2348.80 |
pCO2, HCO3 −, CO3 2−, CO2 and dissolved inorganic carbon (DIC) were calculated based on TA and pH using CO2sys.