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. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857

Figure 8. Detection of ex vivo-derived oligomeric assemblies.

Figure 8

(A) CSF samples from 10 PD patients having a clinical stage of disease around 2 according to the Hoehn and Yahr scale [35] and 10 age-matched controls were probed for the presence of α-synuclein oligomers using a homopaired sandwich ELISA based on the ASyO2 antibody. The age of each individual is given below the corresponding bar. The concentration of α-synuclein oligomers was determined using a standard curve of in vitro α-synuclein oligomers based on their monomeric protein concentration. (B) A sandwich ELISA setup using mAB-O and mAB-M to evaluate binding to Aβ oligomers derived from the well-established and previously described 7PA2 cells [38].