Table 1. Annotated transcripts differentially expressed in AMØs, MoDCs and BMCs at 0 h, 1 h and 24 h p.i. following PCV2b infection.
Cell type | Time-point p.i. | Gene symbol | FC | Gene description |
AMØs | 0 h | PRDX6 | 2.58 | Peroxiredoxin-6 |
1 h | TNF | 2.32 | Tumor necrosis factor | |
MoDCs | 1 h | IL1B | 6.47 | Interleukin 1-beta |
CXCL2 | 4.66 | Chemokine (C-X-C motif) ligand 2 | ||
IL8 | 4.4 | Interleukin 8 | ||
TNF | 4.17 | Tumor necrosis factor | ||
IER3 | 3.4 | immediate early response 3 | ||
CCL4 | 3.37 | Chemokine (C-C motif) ligand 4 | ||
ADM | 3.27 | adrenomedullin | ||
IL1A | 3.2 | Interleukin-1 alpha | ||
MAP3K8 | 2.91 | Mitogen-activated protein kinase 8 | ||
BTG2 | 2.35 | B-cell translocation gene 2 | ||
RND3 | 2.29 | rho Family GTPase 3 | ||
JAG1 | 2.15 | jagged 1 | ||
TNFAIP3 | 2.14 | tumor necrosis factor, alpha-induced protein 3 | ||
IRF1 | 2.1 | interferon regulatory factor 1 | ||
NFKBIA | 2.08 | NF-kappa-B inhibitor alpha | ||
IRG1 | 2.01 | immune-responsive gene 1 protein homolog | ||
MFSD2A | 1.98 | Major facilitator superfamily domain-containing protein 2 | ||
OSM | 1.86 | oncostatin M | ||
HBEGF | 1.84 | heparin-binding EGF-like growth factor | ||
CCL20 | 1.82 | chemokine (C-C motif) ligand 20 | ||
STAB1 | 1.82 | stabilin 1 | ||
TOB1 | 1.73 | transducer of ERBB2, 1 | ||
SLC2A6 | 1.71 | solute carrier family 2 (facilitated glucose transporter), member 6 | ||
CSRNP1 | 1.68 | cysteine-serine-rich nuclear protein 1 | ||
MoDCs | 24 h | VDAC1P5 | 4.06 | voltage-dependent anion channel 1 pseudogene 5 |
S100A9 | 3.3 | S100 calcium binding protein A9 | ||
COX3 | 3.21 | cytochrome oxidase 3 | ||
ARHGAP25 | 2.85 | Rho GTPase activating protein 25 | ||
SLC25A6 | 2.71 | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 | ||
RPL32 | 2.46 | ribosomal protein L32 | ||
MRPL18 | 2.28 | mitochondrial ribosomal protein L18 | ||
RTN4 | 2.25 | reticulon 4 | ||
TGH2 | 2.17 | tissue transglutaminase homologue | ||
PDLIM1 | 2.1 | PDZ and LIM domain 1 | ||
PLCXD1 | 1.99 | phosphatidylinositol-specific phospholipase C, X domain containing 1 | ||
CASP10 | 1.78 | caspase 10, apoptosis-related cysteine peptidase | ||
RANBP1 | 1.77 | RAN binding protein 1 | ||
VDAC2 | 1.66 | voltage-dependent anion channel 2 | ||
FCN1 | −1.75 | ficolin (collagen/fibrinogen domain containing) 1 | ||
BTC | −1.77 | betacellulin | ||
ITGB5 | −2.03 | integrin, beta 5 | ||
PIK3IP1 | −2.05 | phosphoinositide-3-kinase interacting protein 1 | ||
SEPP2 | −2.08 | septin 2 | ||
RNASE4 | −2.27 | ribonuclease, RNase A family, 4 | ||
DKK3 | −2.29 | dickkopf 3 homolog | ||
DUOXA2 | −3 | dual oxidase maturation factor 2 | ||
BMCs | 0 h | PLDN | 1.56 | pallidin homolog |
BMCs | 24 h | Es25 | 3.9 | Esterase 25 |
IGSF8 | 2.78 | immunoglobulin superfamily, member 8 | ||
PTGES | 2.54 | prostaglandin E synthase | ||
TMEM237 | −1.6 | transmembrane protein 237 | ||
MMRN2 | −1.72 | multimerin 2 | ||
MYL1 | −2.27 | myosin, light chain 1 | ||
PEG10 | −2.57 | paternally expressed 10 |
A total of 93 transcripts showed differential expression. Annotated transcripts are listed in each immune cell subset from the highest to the lowest fold-change at the different time points p.i.