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. 2014 Mar 11;9(3):e91325. doi: 10.1371/journal.pone.0091325

Figure 2. Clonogenic assays on NPC cells treated in vitro with Abexinostat combined with CDDP.

Figure 2

The experiments corresponding to the most successful combinations of drug dosages are presented for HONE1, CNE1 and C666-1. Cells were plated as explained in the Materials and Methods section. Simultaneous treatment by Abexinostat and CDDP was started 24 hours following cell plating. About 3 weeks later, cell clones were numbered for calculation of the percentages of growth inhibition (which are mentioned under the picture of each well). For C666-1 cells treated with Abexinostat by itself (40 nM), clonal growth was slightly enhanced instead of being inhibited. The “excess over Bliss additivism” (EOBA) which reflects the level of synergy for the combined treatment is mentioned for each NPC model (see the Materials and Methods section for the explanation of this index). Values greater than 10 are indicative of a synergy between the 2 compounds. The panel of each NPC model is representative of 3 similar experiments.