Figure 4. Top-2 most significant protein networks of differentially regulated proteins following CHIKV infection.

Ingenuity Pathway Analysis of the total 177 proteins identified as differentially expressed generated 2 emerging networks with high score and including more than 20 molecules with direct relationships. Network 1 (A,B,C) was associated with Cell Morphology, Tissue Morphology, Infectious disease and Network 2 (D,E,F) with Cell-To-Cell Signaling and Interaction, Cellular Assembly and Organization, Cellular Compromise. Each network was overlaid with the protein expression fold-change determined in each separate comparison (early (E) vs mock (M) (A, D), late paralytic (LP) vs E (B, E) or late tetanus-like (LT) vs E (C, F)), to highlight the proteins modified during the time-course of infection. Individual proteins are represented as nodes colored in red and green corresponding to up- and down-regulated proteins, respectively, while the nodes (proteins) in white have been added by IPA to maximize the network connectivity. The different shapes of the nodes represent functional classification of the proteins as indicated in the legend.