Figure 6. ROS production and enzymatic activities of NE and MPO in tachyzoite-exposed bovine PMN.

Bovine PMN were exposed to B. besnoiti tachyzoites, zymosan (positive control) or plain medium (negative control) for 30 min. Thereafter, enzymatic activities of NE and MPO as well as ROS production were measured in the supernatants via the NE-chromogenic substrate MeoSuc-Ala-Ala-Pro-Val-chloromethyl ketone, Amplex red and the oxidation of DCFH-DA to fluorescent DCF, respectively. All experiments were performed in triplicates. Arithmetic means of three PMN donors, minimum and maximum. Differences were regarded as significant at a level of p≤0.05.