Figure 8. NET formation induced by differentially treated tachyzoites.

Bovine PMN were exposed to vital, UV- attenuated, homogenized, and heat-inactivated B. besnoiti tachyzoites for 90 min. Stimulation with zymosan (1 mg/ml) served as positive control, plain medium was used as negative control. Samples were analysed for extracellular DNA by quantifying Pico Green-derived fluorescence intensities. Each condition was performed in triplicates. Arithmetic means of three PMN donors, minimum and maximum. Differences were regarded as significant at a level of p≤0.05.