Figure 1. Acute ethanol causes widespread mitochondrial depolarization in the liver.

Mice were treated with saline (left column) or ethanol (6 g/kg, i.g., right column). Intravital multiphoton microscopy of Rh123 was performed after 6 h using a 25× water objective lens. A and D, zooming at 0.7×. B, C, E and F, zooming at 4×. White arrows identify zooming areas shown in B and E. Red arrows identify zooming areas shown in C and F. Darker areas are pericentral regions of the liver lobules. Bar is 100 µm in A and D and 10 µm in B, C, E and F.