Figure 2. Ethanol causes reversible mitochondrial depolarization in vivo in a dose-dependent manner.

Mice were gavaged with one dose of ethanol (0–6 g/kg) in saline, and mitochondrial polarization was detected by intravital multiphoton microscopy of Rh123 at 0 to 24 h after treatment. Representative images from mice treated with 6 g/kg ethanol are shown in A–C. Bar is 10 µm. D: time course of mitochondrial depolarization after treatment with 6 g/kg ethanol; E: dose-dependency of mitochondrial depolarization at 6 h after ethanol treatment. Values are means ± SEM (n = 4–5 per group). *, p<0.05 vs no ethanol.