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. 2014 Mar 11;9(3):e91331. doi: 10.1371/journal.pone.0091331

Figure 2. Expression of heat shock protein 27 in NSCLC patients.

Figure 2

(A) Lung samples (lung cancer and corresponding normal adjacent lung tissues) were analyzed with antibody to heat shock protein 27 (HSP27) in immunohistochemical staining (DAB staining and hematoxylin counterstaining). For negative controls, the antibody was replaced by control IgG. HSP 27 was overexpressed in NSCLC tissue than normal lung tissue. (B) Western blotting analysis of HSP 27 expression in NSCLC tissue and normal tissue. Representative data from four different patients with NSCLC are shown (T =  tumor; N =  normal). The expression of HSP27 protein expression was significantly increased in NSCLC tissue as compared to normal lung tissue (*p<0.05).