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. 2014 Mar 11;9(3):e91508. doi: 10.1371/journal.pone.0091508

Figure 4. Reverse of inhibitory effect of thiacremonone by transfection of mutant prdx6 (C47S).

Figure 4

(A) Lung cancer cells were transfected with pcDNA-prdx6 or pcDNA-prdx6 C47S, and then, thiacremonone was treated (50 μg/ml) for another 72 hrs. The cells were harvested by trypsinization and stained with 0.2% trypan blue. (B) Cell extracts were analyzed by western blotting. Each image and band is representative of three independent experiments. (C) The levels of glutathione peroxidase activity in lung cancer cells were measured using assay kits, as described in Materials and methods. Values are mean ±S.D. #, p<0.05 significantly different from untreated control cells. *, p<0.05, significantly different from untreated control cells transfected with pcDNA-prdx6 C47S. &, p<0.05, significantly different between pcDNA-prdx6 and pcDNA-prdx6 C47S treated with thiacremonone.