Table 1. Characteristics of 13 ponds, and levels of microsatellite DNA and mtDNA variation for S. crocodilurus.

pond	Locality	Latitude (N)	Longitude (E)	sample sizes	HO	HE	FIS	S	h	π	Tajima’s D	P-value
DYSY	Jinxiu, Guangxi, China	24°1′34″	110°14′57″	12	0.57	0.64	0.13	1	0.00	0.0000	0.00	1.000
DCC	Hezhou, Guangxi, China	24°05′56.06″	111°48′13.91″	20	0.40	0.62	0.30	1	0.00	0.0000	0.00	1.000
CSC	Hezhou, Guangxi, China	24°05′44.79″	111°48′32.35″	14	0.32	0.52	0.37	2	0.33	0.0094	−1.52	0.001
DSC	Hezhou, Guangxi, China	24°04′55.57″	111°49′41.88″	10	0.41	0.68	0.42	4	0.73	0.0008	0.00	1.000
YSC	Hezhou, Guangxi, China	24°05′54.79″	111°48′21.08″	24	0.33	0.58	0.40	1	0.00	0.0000	0.00	1.000
GP	Guiping, Guangxi, China	23°33′8″	109°57′39″	9	0.46	0.70	0.35	1	0.00	0.0000	0.00	1.000
GPY	Guiping, Guangxi, China	23°44′19″	110°5’39″	15	0.33	0.68	0.52	1	0.00	0.0000	0.00	1.000
MBC	Luokeng, Guangdong, China	24°34′30″	114°16′50″	14	0.34	0.67	0.50	2	0.33	0.0004	1.03	0.897
CSK	Luokeng, Guangdong, China	24°34′10″	113°16′29″	7	0.45	0.37	0.37	2	0.40	0.0003	−0.97	0.202
DBT	Luokeng, Guangdong, China	24°36′50″	113°21′12″	12	0.37	0.62	0.40	1	0.00	0.0000	0.00	1.00
SK	Luokeng, Guangdong, China	24°35′08″	113°21′14″	7	0.42	0.58	0.29	1	0.00	0.0000	0.00	1.00
LZD	Maoming, Guangdong, China	22°20′	111°29′	40	0.42	0.73	0.41	3	0.80	0.0017	0.96	0.782
LHD	Maoming, Guangdong, China	22°21′57.68″	111°24′46.93″	32	0.51	0.71	0.25	4	0.80	0.0021	0.23	0.621

Expected heterozygosity (H_E), Observed herterozygosity (Ho) and inbreeding coefficients (F_IS) are means across all loci. The haplotypes found in each pond are given along with haplotype diversity (h) and nucleotide diversity (π), (S: number of haplotypes observed) and Tajima’s D with its associated P-value. Population abbreviations are the same as those in Figure 1.