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. 2014 Mar 11;9(3):e91530. doi: 10.1371/journal.pone.0091530

Figure 3. Fluoxetine treatment following chronic CORT exposure reverses the persistent enhancement in memory-related IEG expression in the LA.

Figure 3

(A) Schematic of the behavioral protocol. Rats received either Water or CORT (50 μg/ml) for 2 weeks followed by CORT titration (25 μg/ml, 12.5 μg/ml). Rats then received 3 weeks of either Water or fluoxetine (FLX; 333 μg/ml) and were then sacrificed. (B) Mean (±SEM) immunoreactivity for Arc/Arg3.1 (Water/Water: n = 8; Water/Flx: n = 8; CORT/Water: n = 8; CORT/Flx: n = 7) and Egr-1 (Water/Water: n = 8; Water/Flx: n = 8; CORT/Water: n = 8; CORT/Flx: n = 8) in the LA for each group. (C) Mean (±SEM) immunoreactivity for GluR1 and synaptophysin (Water/Water: n = 8; Water/Flx: n = 8; CORT/Water: n = 8; CORT/Flx: n = 8) in the LA for each group. Representative Western blots for all proteins are shown in the inset. For each experiment, protein levels have been normalized to GAPDH levels for each sample and expressed as a percentage of the Water/Water group. (*) p < 0.05 relative to Water/Water group.