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. 2014 Mar 11;9(3):e91794. doi: 10.1371/journal.pone.0091794

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© 2014 Shi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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rMjGal, Trx-His, or PBS were injected into shrimp and the hemocytes were collected and analyzed by immunohistochemistry. The left panel indicated the binding signal detected by the anti-Histidine antibody for the recombinant protein (Green), the middle panel showed the hemocyte nucleus location (Blue), and the right panel showed the merge of previous two panels. Bar = 20 µm.