Table 1. Hypoxic phenotypes of selectively targeted strains.
| Phenotype | Recovery timea |
Genotypes |
|||
|---|---|---|---|---|---|
| rars-1(gc47) | gc47;gcSi2 | gc47;gcIs3 | gc47;gcSi4 | ||
| Pumping (pumps/min) | 24 h | 163±40 (50.5) | 159±54 (50.5) | 18±44 (54.5) | 14±26 (30.3) |
| Unc (%) | 72 h | 8±10 (397.7) | 7±9 (355.7) | 40±11 (423.7)c | 29±6 (246.4)c |
| Velocity (mm/s) | 72 h | 0.16±0.050 (30.3) | 0.15±0.046 (30.3) | 0.01±0.015 (30.3)c | 0.01±0.012 (30.3)c |
| Body bend/min | 48 h | 18.1±2.43 (10.1) | 15.4±3.30 (20.2) | 0.4±1.32 (40.4)c | 0.7±1.59 (10.1)c |
| Death (%) | 96 h | 2.8±3.58 (397.7) | 2.4±3.79 (355.7) | 57.3±23.68 (423.7) | 39.0±18.85 (246.4) |
| PID (%) | 96 h | 0 (508.8) | 0 (449.8) | 26.9±13.74 (545.8) | 27.3±14.41 (259.4) |
| Pharyngeal cell swelling (%) | 72 h | 6.0±8.94 (100.5) | 10.0±13.22 (60.3) | 48.3±14.43 (60.3) | 51.7±15.28 (60.3) |
| Tail cell swelling (%) | 72 h | 6.0±6.52 (100.5) | 3.3±5.77 (60.3) | 35.0±5.00 (60.3) | 43.3±14.33 (60.3) |
| GABA neuropathology(%)b | 48 h | 11.7±5.77 (60.3) | 0 (60.3) | 60.0±15.00 (60.3) | 70.0±13.23 (60.3) |
Abbreviations: GABA, gamma-aminobutyric acid; Unc, uncoordinated; PID, pharyngeal intestinal detachment
a
Recovery from a 21 h hypoxic insult
b
GABA neuron visualized by a transgene oxIs12 [Punc-47::GFP]
C
Severe Unc (uncoordinated) animals scored. Data are mean±S.D.(no. of animals, no. of trials)