Figure 4.

PPARγ2 is regulated by Fra-2 and can regulate HIF expression. (a) Real-time PCR analyses of Fabp4, PPARα and PPARγ2 in fat pad tissue from Fra-2^Δadip mice and littermate controls at 6 weeks after tamoxifen injections with mice receiving normal diet (ND) or high-fat diet (HFD) (n=4–5). (b) Real-time PCR analyses of Fabp4 and PPARγ2 in adipocytes of Fra-2^Δadip and controls cultures at days 0 and 7 of in vitro differentiation (n=3). (c) Oil Red O staining of adipocytes derived from fat pad tissue of Fra-2^Δadip mice and littermate controls at day 7 of in vitro differentiation. Bars represent 200 μm. Arrows indicate adipocytes. (d and e) Real-time PCR analyses of PPARγ2, Fabp4 (d) and HIF1α, HIF1β and HIF2α (e) in primary adipocytes treated with vehicle or BADGE (n=3). Bars represent mean values±S.D. Statistical analyses: *P<0.05, **P<0.01, ***P<0.001