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. 2014 Mar 1;28(5):479–490. doi: 10.1101/gad.231233.113

Figure 1.

Figure 1.

Generation and characterization of conditional mutant IDH2 mice. (A) Schematic for the adult IDH2 mutant mouse model generated from crosses of Ubiquitin CreERT2 and Lox-stop-lox IDH2. (B–F) Mice of the indicated genotypes were examined for serum 2HG levels measured using the 2HG standards at 4 wk after tamoxifen administration (B), Kaplan-Meier survival analysis (C), gross appearance and H&E staining of the heart (D), heart weight (n = 5, 6, and 6 mice, respectively) (E), and heart 2HG levels (F), presented in relative arbitrary units. (G–I) Mice with embryonic activation of IDH2 R140Q (progeny from crossing Actin Cre and LSL-R140Q mice) and controls examined for gross appearance at 7 and 21 d (G), body weight and heart weight at ages 3 wk and 6 wk, respectively (n = 3 for both groups for both graphs) (H), and survival (n = 8 and 6 for Actin Cre LSL-R140Q and controls, respectively) (I).