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. 2014 Mar 1;28(5):502–520. doi: 10.1101/gad.236547.113

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© 2014 Dong et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 4. — C3:G70 in the acceptor stem is crucial for accurate AUG selection and rapid TC binding to PICs in vivo. (A) Acceptor stem structures and phenotypes (in order of severity: Sui³⁻ > Sui²⁻ > Sui⁻ > Sui^−/+) for 3:70 substitutions. (B) Slg⁻ phenotypes on +His medium analyzed as in Figure 1C. (C) His⁺/Sui⁻ phenotypes analyzed as in Figure 2B. (Results in B and C were obtained in parallel from the same plates and rearranged only for ease of interpretation.) (D) UUG:AUG initiation ratios were determined as in Figure 1B. (E) β-Galactosidase expressed from the GCN4-lacZ reporter on p180 measured as in Figure 1B.