Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 1;28(5):521–531. doi: 10.1101/gad.233916.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Tagami et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

Figure 4. — The mechanism of transcription inhibition by gp39. (A,B) Superimposition of holo•gp39 and the free holoenzyme (Protein Data Bank [PDB] 1IW7; green) (Vassylyev et al. 2002). (A) The core modules of the two RNAP structures were superimposed by minimizing the root mean square deviation (RMSD) between the Cα atoms. (B) Superimposition of the σ₄ domains of the two structures. (C) Transcription inhibition by wild-type gp39 (wt) and its truncated variant lacking the C-terminal tail (1–113) on a −10/−35 promoter (T5 N25). (D) Analysis of transcription inhibition by gp39 added either before (lane 2) or after (lane 3) the promoter DNA. Black triangles indicate order of addition. (E) Analysis of gp39 (wild type [wt] and 1–113) on promoter melting by potassium permanganate probing. Thymine positions in the melted promoter region (numbered relative to the transcription starting point) are indicated on the right.