Figure 1. Cardiac Specific Deletion of ILK.
Total RNA and protein were isolated from whole ventricles of 10 day old CSILK-KO and control littermates. (A) ILK mRNA expression was determined by QRT-PCR, *p < 0.05, n=5 in each genotype. (B) ILK protein expression was determined by immunoblotting. Results shown are representative of three independent experiments. (C) Immunofluorescence confocal microscopy shows virtually absent ILK staining in CSILK-KO cardiomyocytes identified by α-actinin staining but preserved vascular expression. Representative results from three independent experiments are shown.