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In vitro perfusion of ovine intact ovary with freezing medium. ((a), (b)) Aorta with arteria ovarica (24 G catheters inside) and ((c), (d), and (e)) perfusion with freezing medium: 3 min (c), 5 min (d), and 10 min (e) elapsed from the beginning of perfusion, vena ovarica (white arrows), ovary (black arrow). Freezing medium includes Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum + 6% dimethyl sulfoxide + 6% ethylene glycol + 0.15 M sucrose + Indian ink. Bar = 1 cm.
