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End of perfusion of ovine intact ovary with vascular pedicle with freezing medium. ((a), (b)) Ovary after successful perfusion (~95% of tissues excluding yellow body) and (c) ovary after unsuccessful perfusion (~10% of tissues), yellow body (arrows). Note the unsuccessful perfusion of yellow body. Freezing medium includes Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum + 6% dimethyl sulfoxide + 6% ethylene glycol + 0.15 M sucrose + Indian ink. Bar = 0.5 cm.
