In situ Hybridization. ISH in p5 postnatal rat whole-body sections stained with cresyl violet (a). Emulsion autoradiography bright ISH labeling is seen in (b) WT rat and (c) TRPC4+/− (Het) but not (d) TRPC4−/− (Hom). Control (sense) ISH is in (e).
Abbreviations: Br – brain; Cb – cerebellum; Ch – choroid; Cx – brain cortex; E – eye; H – heart; Hi – hippocampus; In –intestine, Inc – incisor; Het – heterozygous (TRPC4+/−); Hom – homozygous (TRPC4−/−); KO – knockout (TRPC4−/−); Li – liver, erythropoietic centers; R – ribs; SC – spinal cord; St – Stomach; Th – thymus; (as) – antisense; (cv) – cresyl violet; (s) – sense. The asterisks (*) indicate non-specific hybridization in the eye choroid (*) and dorsal aorta (**).