(A) Pre-treatment. TRPC4 antagonist ML-204, dose-dependently inhibited MO-induced pain-related behaviors in wild type rats injected intraperitoneally.
(B) Post-treatment. ML-204 inhibited pain-related behaviors induced by intracolonic MO infusion. Values represent mean ± SED (n=4, 1 hr).
(C) Pre-treatment. ML-204 inhibited mechanical allodynia induced in WT rats 30 minutes after intracolonic MO infusion.
(D) Responses to morphine. Inhibition of pain-related behaviors induced by intracolonic MO infusion after subcutaneous pre-treatment with morphine.
(*p<0.05, **p<0.01 vs. vehicle)
Abbreviations: PO vehicle–peanut oil; MO- mustard oil irritant