Table 1.
Targets | Sequence | Length (bp) | Annealing (°C) | Accession |
---|---|---|---|---|
β-actin | ||||
fwd1 | TGTTACCAACTGGGACGACA | 165 | 58 | NM_007393.3 |
rev2 | GGGGTGTTGAAGGTCTCAAA | |||
Bglap3 | ||||
fwd | TTCTGCTCACTCTGCTGACC | 111 | 58 | NM_007541.2 |
rev | TATTGCCCTCCTGCTTGGAC | |||
ctsK4 | ||||
fwd | GAGGCGGCTATATGACCACT | 119 | 58 | NM_007802.3 |
rev | CTTTGCCGTGGCGTTATACA | |||
col15 | ||||
fwd | TGGCATCCCTGGACAGCCTG | 144 | 62 | NM_007742.3 |
rev | ATGGGGCCAGGCACGGAAAC |
1Forward; 2reverse; 3bglap: gene name of osteocalcin; 4cathepsin K; 5collagen 1α1.