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. 2014 Feb 4;5(1):e01102-13. doi: 10.1128/mBio.01102-13

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Copyright © 2014 Josset et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 1 — H7N9 replicates to a level similar to that of other influenza A viruses (IAVs) in polarized human bronchial epithelial Calu-3 cells. Polarized Calu-3 cells were infected apically with Anhui01 (H7N9), NL219 (H7N7), Pan99 (H3N2), or VN1203 (H5N1) at an MOI of 1 for an hour. After washing, medium was added and supernatants were collected at 3, 7, 12, and 24 h for determination of viral titers by standard plaque assay. Cells were harvested at the same time postinfection for transcriptomic profiling. Values represent means of titers in PFU/ml from quadruplicate wells ± SD.