Table 2.
LTA4H activity (U) in the plasma, synovial fluid (SF), soluble (SO), and solubilized membrane-bound (MB) fractions of synovial tissue (ST) and peripheral blood mononuclear cells (PBMCs) from control, CIA-arthritic, and CIA-resistant rats.
| Samples | Control | CIA-arthritic | CIA-resistant | ANOVA |
|---|---|---|---|---|
| PLASMA | 20.89 ± 0.77 | 28.92 ± 3.33 | 29.24 ± 3.77 | P = 0.1540 |
| SF | 12.66 ± 0.86a | 21.16 ± 1.34b | 44.91 ± 3.00c | P < 0.0001 |
| SO-ST | 57.72 ± 8.20 | 38.81 ± 6.83 | 52.43 ± 4.61 | P = 0.2023 |
| MB-ST | 13.85 ± 0.03a | 0 ± 3.45a | 56.29 ± 15.10b | P = 0.0060 |
| SO-PBMCs | 0 ± 12.48a | 21.76 ± 10.01b | 753.72 ± 5.23c | P < 0.0001 |
| MB-PBMCs | 142.30 ± 31.50a | 57.42 ± 14.53b | 48.86 ± 0.62b | P = 0.0306 |
Values are mean ± SEM. U = LTA4H unit = LTA4H activity in 1 mL sample that catalyzes the formation of 1 picomole of LTB4 per minute. Number of animals = 3, values of each animal measured in duplicate. Student-Newman-Keuls, P < 0.05: comparison among control versus CIA-arthritic versus CIA-resistant in the same row (different letters indicate significant difference).